DNA Polymerases Used in PCR

Question

DNA polymerases used in PCR
A)use an RNA template to make complementary DNA.
B)must remain active at very cold temperatures.
C)include Taq polymerases and Vent polymerase.
D)are labeled with fluorescent dyes.
E)All of these choices are correct.

Accepted Answer

DNA polymerases used in PCR include Taq polymerases and Vent polymerase. Choice A is incorrect because DNA polymerases use a DNA template, not RNA. Choice B is incorrect because Taq polymerase, one of the most commonly used polymerases in PCR, is active at high temperatures, not cold temperatures. Choice D is incorrect because fluorescent dyes are not typically used to label DNA polymerases in PCR.