Gene Probes Can Be Labeled for Detection With

Question

Gene probes can be labeled for detection with
A)enzymes.
B)fluorescent dyes.
C)radioisotopes.
D)All of these choices are correct.

Accepted Answer

All of the listed choices are commonly used to label gene probes for detection. Enzymes (such as alkaline phosphatase or horseradish peroxidase) can be conjugated to probes and detected via colorimetric or chemiluminescent assays. Fluorescent dyes (such as Cy3 or Texas Red) can be directly coupled to probes and visualized using fluorescence microscopy or flow cytometry. Radioisotopes (such as 32P or 35S) can be incorporated into probes and detected via autoradiography or scintillation counting. Each labeling method has its advantages and disadvantages, and the choice often depends on the specific application and available technology.