Which Procedure Would You Use to Detect a Nutritional Mutation

Question

Which procedure would you use to detect a nutritional mutation in microorganisms?
A)Visible inspection
B)Replica plating
C)Controlled crosses
D)Isolation at high temperature
E)Plating on antibiotic-containing medium

Accepted Answer

Replica plating is a technique used to transfer bacterial colonies from one plate to another. It can be used to screen for mutants that have lost the ability to synthesize certain nutrients. A master plate is made with wild type bacteria, and then replica plates are made on different types of medium. Mutant colonies can be identified by observing their growth or lack of growth on specific medium.