In Recombinant DNA Techniques,the Gaps in the Paired DNA Fragments

Question

In recombinant DNA techniques,the gaps in the paired DNA fragments are closed by ____.
A) restriction enzymes
B) DNA ligase
C) DNA polymerase
D) DNA replicase
E) reverse transcriptase

Accepted Answer

The gaps in paired DNA fragments are closed by DNA ligase, an enzyme that catalyzes the formation of phosphodiester bonds between the sugar-phosphate backbone of DNA fragments. Restriction enzymes are used to cut DNA at specific sites, DNA polymerase is used to synthesize new DNA strands, DNA replicase is a type of DNA polymerase involved in replication, and reverse transcriptase is an enzyme used to generate cDNA from mRNA. None of these enzymes are specifically used to close gaps in paired DNA fragments.