Dolly,the Famous Sheep,was Cloned Using

Question

Dolly,the famous sheep,was cloned using:
A)induced pluripotent stem cells.
B)homologous recombination.
C)somatic cell nuclear transfer.
D)Cre recombinase.

Accepted Answer

Dolly was cloned using somatic cell nuclear transfer, where the nucleus of an adult somatic cell was transferred into an enucleated egg cell. This process created an embryo that was implanted into a surrogate mother, leading to the birth of Dolly. Induced pluripotent stem cells and homologous recombination are also methods used in genetic engineering, but they were not involved in the cloning of Dolly. Cre recombinase is an enzyme used in genetic engineering to manipulate gene expression and excise specific DNA sequences, but it was not used in the cloning of Dolly.