In a cell-free protein synthesis system utilizing microsomes from fragmented ER,under which condition could you determine if the new protein was imported into the microsome?

A)Ribosomes and mRNA are incubated with microsomes,then a protease is added and the results are analyzed.
B)Ribosomes and mRNA are incubated with microsomes,then a protease and detergent are added and the results are analyzed.
C)Ribosomes and mRNA are incubated with protease,then microsomes are added and the results are analyzed.
D)Ribosomes and mRNA are incubated with microsomes,then detergent is added and the results are analyzed.