Question 3

(Essay)

Describe how you would prepare liposomes from a biological membrane.

Answer

The first step would be to treat the biological membrane with an organic solvent such as a mixture of chloroform and methanol.This would dissolve phospholipids and cholesterol,but not proteins or carbohydrates.Once the dissolved lipids were separated from the proteins and carbohydrates,you would evaporate the solvent.The final step would be to disperse the residue (which consists of phospholipids and cholesterol)in water to form liposomes.