Which of the Following Enzymes Can Permanently Remove Supercoiling from Circular

Question

Which of the following enzymes can permanently remove supercoiling from circular DNA molecule?
A) Histones
B) Restriction endonuclease
C) DNA ligase
D) Topoisomerase I
E) DNA gyrase

Accepted Answer

Topoisomerase I is an enzyme that can permanently remove supercoiling from a circular DNA molecule by making a single-strand break in one of the DNA strands, allowing the DNA to relax and unwind, and then rejoining the strands. Histones, restriction endonucleases, DNA ligase, and DNA gyrase do not have the ability to remove supercoiling from a circular DNA molecule.