454 Pyrosequencing Coupled Advances In

Question

454 pyrosequencing coupled advances in:
A)DNA sequencing and DNA amplification
B)DNA sequencing and DNA cloning
C)DNA cloning and DNA amplification
D)Radiolabeling and DNA cloning
E)Fluorescence labeling and DNA cloning

Accepted Answer

Pyrosequencing involves detecting nucleotide incorporation during DNA synthesis, which requires amplification of the DNA sample. Thus, Pyrosequencing is coupled with both DNA sequencing and DNA amplification. Cloning and radiolabeling are not directly related to Pyrosequencing, and fluorescence labeling is not necessarily always used in Pyrosequencing.