The Sanger Method of DNA Sequencing Has Been Automated and Uses

Question

The Sanger method of DNA sequencing has been automated and uses a(n):
A)thermal-stable DNA polymerase to allow for multiple rounds of DNA synthesis.
B)trideoxynucleotides in the synthesis reactions.
C)none is a correct choice
D)agarose gel electrophoresis for fragment separation.
E)chemical digestion method for fragment generation.

Accepted Answer

The Sanger method uses a thermal-stable DNA polymerase to perform multiple rounds of DNA synthesis in automated sequencing, allowing for the incorporation of labeled dideoxynucleotides for chain termination.