The Following Steps are Used to Produce Monoclonal Antibodies

Question

The following steps are used to produce monoclonal antibodies.What is the fourth step?
A)A B cell is activated to produce antibodies.
B)Culture the hybridoma in a selective medium.
C)Fuse a B cell to a myeloma cell.
D)Isolate antibody-producing B cells.
E)Vaccinate a mouse.

Accepted Answer

The fourth step in producing monoclonal antibodies is to fuse a B cell to a myeloma cell to create a hybridoma. This hybrid cell will have the ability to produce a specific antibody while also having the immortal growth properties of the myeloma cell. Once the hybridoma is created, it can be cultured and cloned to produce large quantities of the desired monoclonal antibody.