The Use of an Antibiotic-Resistance Gene on a Plasmid Used

Question

The use of an antibiotic-resistance gene on a plasmid used in genetic engineering makes
A)replica plating possible.
B)direct selection possible.
C)the recombinant cell dangerous.
D)the recombinant cell unable to survive.
E)All of the answers are correct.

Accepted Answer

The use of an antibiotic-resistance gene on a plasmid used in genetic engineering allows for direct selection of cells that have taken up the plasmid. Cells that have not taken up the plasmid will not have the antibiotic resistance and will die, while cells that have taken up the plasmid will survive due to the resistance gene. This allows for easy selection and enrichment of the desired recombinant cells.