Which of the Following is a Limitation of the Autoclave

Question

Which of the following is a limitation of the autoclave?
A)It requires an excessively long time to achieve sterilization.
B)It cannot inactivate viruses.
C)It cannot kill endospores.
D)It cannot be used with heat-labile materials.
E)It cannot be used with glassware.

Accepted Answer

Autoclaves use steam under pressure to achieve sterilization, effectively killing bacteria, viruses, fungi, and spores. However, they cannot be used with materials that are sensitive to heat (heat-labile), as the high temperatures would damage or alter these materials.