Which of the Following Statements Regarding the Use of CRISPR/Cas

Question

Which of the following statements regarding the use of CRISPR/Cas in gene editing is FALSE?
A) The guide RNA is complementary to the DNA sequence to be edited
B) The guide RNA associates with the introduced foreign DNA, "guiding" it to the spot where it will be inserted into the host DNA
C) The host DNA to be edited is cleaved at a precise spot by Cas
D) Host DNA repair mechanisms are used to fill in breaks between the host DNA and the introduced DNA

Accepted Answer

The guide RNA does not associate with introduced foreign DNA to guide it to the insertion spot. Instead, it directs the Cas enzyme to the specific DNA sequence to be edited by being complementary to that sequence, where the Cas enzyme then makes a cut.