DNA Extraction

Question

DNA extraction
A)uses restriction enzymes to prevent chromosomes from unraveling.
B)can only occur when the gene of interest has been amplified by PCR.
C)occurs only in prokaryotic cells because they lack a protective nucleus.
D)requires that all non-DNA organic compounds be removed with chemicals.

Accepted Answer

DNA extraction requires the removal of all non-DNA organic compounds, such as proteins, lipids, and carbohydrates, to obtain pure DNA. This is typically achieved through the use of chemicals, such as ethanol or phenol, which selectively precipitate the DNA while leaving behind other cellular components. Restriction enzymes are not used in the DNA extraction process, and PCR amplification is not always necessary, as DNA can be extracted directly from cells or tissues. DNA extraction can also occur in eukaryotic cells with additional steps required to isolate the DNA from the nucleus.