Which of the Following Most Correctly Describes a Shotgun Technique

Question

Which of the following most correctly describes a shotgun technique for sequencing a genome?
A)genetic mapping followed immediately by sequencing
B)physical mapping followed immediately by sequencing
C)cloning large genome fragments into very large vectors, followed by sequencing
D)cloning several sizes of fragments into various size vectors, sequencing the clone and then ordering them
E)cloning the whole genome directly, from one end to the other

Accepted Answer

The shotgun technique for genome sequencing involves cloning several sizes of fragments into various size vectors, sequencing the clones, and then ordering them. This approach allows for overlapping sequences to be identified and assembled into a complete genome sequence. Physical and genetic mapping can be used as initial steps to guide the sequencing, but the actual sequencing process involves the fragmentation and cloning of the genomic DNA. Cloning the entire genome directly or cloning large fragments into very large vectors would be impractical and technically challenging.