Question 78

(Multiple Choice)

In an experiment,the bacteriophage Lambda genome was cut into fragments using the restriction enzyme Hind III.Hind III cuts the Lambda producing eight different size fragments when separated by gel electrophoresis.Select an observation that best describes a correct aspect of the two processes of restriction digest and gel electrophoresis:


A)When separated on a gel, the pattern of DNA bands will be characteristic of those cut with Hind III, different restriction enzymes will not produce these same fragments.
B)The Hind III sequence is found eight times in the Lambda genome.
C)If an electrical current is not used, eight separate DNA bands would be visible, but they would not be separated as much as when an electrical current is used.
D)Only the restriction enzyme Hind III can be used to cut Lambda DNA, since restriction enzymes are specific to the type of DNA they can cut.
E)Since the Lambda genome was cut by Hind III, the genome must be composed of RNA.

Answer