Which of the Following is in the Correct Order for One

Question

Which of the following is in the correct order for one cycle of polymerase chain reaction (PCR)?
A)Denature DNA; add fresh enzyme; anneal primers; add dNTPs; extend primers.
B)Anneal primers; denature DNA; extend primers.
C)Extend primers; anneal primers; denature DNA.
D)Denature DNA; anneal primers; extend primers.
E)Denature DNA; add fresh enzyme; add dNTPs; anneal primers; extend primers.

Accepted Answer

The correct order for one cycle of PCR is denature DNA, anneal primers, and extend primers. This allows for the separation of the double-stranded DNA, annealing of the primers to the single-stranded DNA, and extension of the primers by the Taq polymerase. Adding fresh enzyme and dNTPs are not necessary at each cycle as they are already present in the reaction mixture. Therefore, option D is the correct order for one cycle of PCR.