A researcher has used in vitro mutagenesis to mutate a cloned gene and then has reinserted this into a cell.In order to have the mutated sequence disable the function of the gene,what must then occur?

A)recombination resulting in replacement of the wild type with the mutated gene
B)use of a microarray to verify continued expression of the original gene
C)replication of the cloned gene using a bacterial plasmid
D)transcription of the cloned gene using cDNA
E)attachment of the mutated gene to an existing mRNA to be translated