Considering the role of Notch and Wnt signaling pathways in epithelial cell differentiation in the mammalian intestine, indicate whether each of the following changes in "minigut" organoids in culture is expected to lead to a higher (H) or lower (L) number of stem cells. Your answer would be a two-letter string composed of letters H and L only, e.g. HH.
( ) RNA interference knockdown of Hes1, an essential downstream effector of the Notch signaling pathway
( ) Deletion of the gene encoding Apc, a gene that is mutated in many colorectal cancers