Which of the following is NOT correct regarding cryoelectron microscopy?

A) It does not require shadowing or negative staining.
B) It involves obtaining images from many-sometimes tens of thousands of-individual molecules that may or may not all be in the same orientation.
C) Subnanometer resolutions (e.g. 0.5 nm or better) can be achieved by this technique.
D) It can only be used to see the exterior surface of molecules and complexes.
E) Atomic models obtained from x-ray crystallography can be fitted into the density envelope obtained from this technique.
Answers