Consider a protein composed of only 21 amino acids encoded by a short ORF that is located between two known genes in a mammalian genome. Due to its length, the ORF had not been annotated as a gene. Which of the following methods can demonstrate that it is indeed a gene that is actively translated to protein?
A) RNA-seq B) RT-PCR C) DNA microarray analysis D) ChIP-seq E) Ribosome profiling