In Sanger sequencing, does each labeled DNA molecule have a fluorescently labeled dideoxyribonucleotide at its 5′ end or its 3′ end? Do the labeled nucleotides lack the 2′ or 3′ hydroxyl group?
A) 3′ end; 2′ hydroxyl group only B) 3′ end; 3′ hydroxyl group only C) 5′ end; 2′ hydroxyl group only D) 5′ end; 3′ hydroxyl group only E) 3′ end; both hydroxyl groups