The compound GDPNP is a GTP analog that can bind to GTPases in the same way as GTP. However, unlike GTP, it cannot undergo the hydrolysis reaction that normally releases Pᵢ. Therefore, …

A) GDPNP and GDP bind to the same conformation of a GTPase.
B) a GDPNP-bound Ras is constitutively active.
C) a GDPNP-bound EF-Tu cannot bind to tRNA molecules.
D) binding of GDPNP to EF-Tu allows multiple cycles of tRNA binding and release without the use of free energy.
E) None of the above.