Anti-Antibodies are Used in Indirect Antibody Assays

Question

Anti-antibodies are used in indirect antibody assays.

Accepted Answer

Anti-antibodies, also called secondary antibodies, are used in indirect antibody assays to detect the presence of a primary antibody that has already bound to the antigen of interest. The secondary antibody recognizes and binds to the primary antibody, which is usually labeled with a visible marker such as a fluorescent or enzymatic tag. This allows for the detection and quantification of the antigen in the sample being tested.