Synthesis of CDNA Requires the Use Of

Question

Synthesis of cDNA requires the use of
A) DNA ligase.
B) agarose.
C) reverse transcriptase.
D) restriction enzymes.
E) fluorescent synthetic nucleotides.

Accepted Answer

Synthesis of cDNA involves the use of reverse transcriptase, an enzyme that synthesizes complementary DNA (cDNA) from RNA templates. DNA ligase is used in DNA cloning and ligation reactions, agarose is used for gel electrophoresis, restriction enzymes are used in molecular biology for cutting DNA and fluorescent synthetic nucleotides are used in DNA sequencing.