Single-Strand DNA Molecules are Fixed to a Glass Slide or Nylon

Question

Single-strand DNA molecules are fixed to a glass slide or nylon membrane for the production of
A) microarrays.
B) DNA clones.
C) real time PCR probes.
D) FISH assays.
E) templates for Sanger sequencing.

Accepted Answer

Single-strand DNA molecules are commonly used in microarray technology, in which they are attached to a solid support such as glass slides or nylon membranes to allow for the study of gene expression levels or the detection of specific genetic variations. This is not usually the case for the other options listed, which typically involve the use of double-stranded DNA or other nucleic acid constructs.