A Procedure Using Both Reverse Transcriptase and Real Time PCR

Question

A procedure using both reverse transcriptase and real time PCR can be used to
A) determine the number of copies of a DNA sequence in a sample.
B) determine the number of copies of an RNA sequence in a sample.
C) produce DNA fragments for cDNA cloning.
D) both determine the number of copies of an RNA sequence in a sample and produce fragments for cDNA cloning.
E) produce RNA fragments for cloning.

Accepted Answer

This procedure, often referred to as RT-qPCR (Reverse Transcription quantitative PCR), uses reverse transcriptase to convert RNA into cDNA, which is then quantified using real-time PCR. This allows for the determination of the number of copies of an RNA sequence in a sample and the production of DNA fragments for cDNA cloning.