In Gram Staining, Ethanol-Acetone is Used as A

Question

In Gram staining, ethanol-acetone is used as a
A) decolorizing agent.
B) counterstain.
C) mordant.
D) drying agent.
E) primary stain.

Accepted Answer

Ethanol-acetone is used as a decolorizing agent in Gram staining. It is used to remove the crystal violet stain from the gram-negative cells, leaving them colorless and allowing the safranin counterstain to color the cells.