How are the Sticky Ends Obtained

Question

How are the sticky ends obtained?
A) by using a different restriction enzyme to cut both DNA fragment and the plasmid
B) by using the same restriction enzyme to cut both DNA fragment and the plasmid
C) by using a restriction enzyme to cut DNA fragment
D) by using a restriction enzyme to cut the plasmid

Accepted Answer

Sticky ends are obtained by using the same restriction enzyme to cut both the DNA fragment and the plasmid, ensuring that the ends of both DNA molecules can anneal to each other due to complementary base pairing.