Which of the Following is Needed in Order to Amplify

Question

Which of the following is needed in order to amplify the DNA for gene X and NOT for gene Y?
A) a heat-stable DNA polymerase
B) restriction endonucleases that cut gene X and not gene Y
C) plasmid vectors that have multiple antibiotic resistance genes
D) some prior knowledge of the sequence of gene X such that appropriate primers can be designed

Accepted Answer

To specifically amplify gene X and not gene Y, it is essential to have prior knowledge of the sequence of gene X to design specific primers. These primers will anneal only to the desired gene X sequences, allowing for its selective amplification through PCR.