What is the Correct Sequence of the Reagents in the Gram

Question

What is the correct sequence of the reagents in the Gram stain technique?
A) safranin, crystal violet, alcohol, iodine
B) alcohol, iodine, crystal violet, safranin
C) crystal violet, iodine, alcohol, safranin
D) iodine, alcohol, safranin, crystal violet

Accepted Answer

The correct sequence for the Gram stain technique is: crystal violet (primary stain), iodine (mordant), alcohol (decolorizer), and safranin (counterstain). This sequence is used to differentiate between Gram-positive and Gram-negative bacteria based on their cell wall properties.