Which of the Following Must Be Accomplished FIRST in Order

Question

Which of the following must be accomplished FIRST in order to conduct RT-PCR?
A) Complementary DNA must be synthesized from double-stranded DNA.
B) Complementary DNA must be synthesized from RNA.
C) Complementary DNA must be synthesized from proteins.
D) Complementary DNA must be synthesized from single-stranded DNA.

Accepted Answer

RT-PCR stands for reverse transcription polymerase chain reaction, which involves the conversion of RNA into complementary DNA (cDNA) before amplification through PCR. Therefore, the first step is the synthesis of cDNA from RNA.