Which of the Following Methods Do NOT Involve Nucleic Acid

Question

Which of the following methods do NOT involve nucleic acid hybridization of a probe to a specimen?
A) RACE-FISH
B) FISH-MAR
C) PhyloChips
D) T-RFLP

Accepted Answer

T-RFLP (Terminal Restriction Fragment Length Polymorphism) involves the digestion of amplified DNA with restriction enzymes and does not rely on nucleic acid hybridization of a probe to a specimen. Instead, it analyzes the length of fragments generated by restriction enzyme digestion.