Using a Host Defective in Proteases is Likely to Be

Question

Using a host defective in proteases is likely to be necessary when
A) engineering a complete metabolic pathway requiring several different enzymes.
B) overproducing proteins.
C) producing a small protein.
D) engineering transgenic animals with immune systems.

Accepted Answer

Producing a small protein in a host defective in proteases is necessary because small proteins are more susceptible to degradation by proteases. Using a host with reduced protease activity can help increase the yield of intact, functional small proteins.