Cloning Vectors Can Be Distinguished from Expression Vectors By

Question

Cloning vectors can be distinguished from expression vectors by
A) carrying ori genes for replication of the cloned sequence.
B) having a multiple cloning site (MCS).
C) having a high copy number per cell.
D) lacking a promoter site upstream of the insertion site.

Accepted Answer

Cloning vectors typically lack a promoter site upstream of the insertion site, while expression vectors have a strong promoter for efficient expression of the cloned gene. The other options - carrying ori genes, having an MCS, and having a high copy number per cell - are common features shared by both cloning and expression vectors.