The Enzyme That Covalently Links Both Strands of a Vector

Question

The enzyme that covalently links both strands of a vector and inserted DNA in molecular cloning is
A) DNA ligase.
B) DNA phosphatase.
C) DNA hydrolase.
D) DNA transferase.

Accepted Answer

DNA ligase is the enzyme that catalyzes the formation of phosphodiester bonds between the nucleotides of the vector and inserted DNA, covalently linking them together to form a recombinant DNA molecule. DNA phosphatase, DNA hydrolase, and DNA transferase are not involved in this process.