Which of the Following is NOT a Common Step in Molecular

Question

Which of the following is NOT a common step in molecular cloning using plasmids?
A) Fragment DNA into small segments.
B) Hybridize DNA sequences with slightly mismatched oligonucleotides.
C) Ligate DNA into vectors.
D) Insert the vectors into a host.

Accepted Answer

Hybridizing DNA sequences with slightly mismatched oligonucleotides is not a common step in molecular cloning using plasmids. The other three steps - fragmenting DNA into small segments, ligating DNA into vectors, and inserting the vectors into a host - are all essential steps in the process of molecular cloning using plasmids.