What is the Most Important Advantage of Pfu Polymerase Over

Question

What is the most important advantage of Pfu polymerase over Taq polymerase?
A) Unlike Taq polymerase, Pfu polymerase functions well at relatively high temperatures.
B) It is from a bacterium, not an archaean, so it is more effective for replicating eukaryotic DNA.
C) Pfu polymerase removes the need for primers during PCR.
D) Unlike Taq polymerase, Pfu polymerase has proofreading activity.

Accepted Answer

The most important advantage of Pfu polymerase over Taq polymerase is that Pfu polymerase has proofreading activity. This means that it can correct errors made during DNA synthesis, resulting in a lower error rate and higher fidelity of DNA replication. Taq polymerase lacks this capability, leading to a higher error rate and lower fidelity in PCR products.