One of the More Formidable Obstacles to Mammalian Gene Cloning

Question

One of the more formidable obstacles to mammalian gene cloning is the presence of
A) introns.
B) exons.
C) repressors.
D) integrators.

Accepted Answer

Introns are non-coding regions of DNA that interrupt the coding regions (exons) in genes of many eukaryotic genomes, including mammals. These introns must be removed during gene transcription in order for the resulting mRNA to be functional. However, they can complicate the process of gene cloning, as they can lead to incomplete or incorrect splicing of the cloned gene sequence.