RNA sensors can be used to detect pathogens such as ZIKA virus. The steps below are steps used in testing for ZIKA virus. Which has the required steps in the correct order? 1- LacZ gene product is detected via a color change.
2- DNA is added to an RNA switch that has a hairpin to block transcription of lacZ.
3- Multiple copies of DNA matching ZIKA are produced.
4- In the presence of the ZIKA-trigger RNA, the "toehold" switch is bound and releases the lacZ rbs and start codon so transcription can occur.
5- Reverse transcriptase is used to produce cDNA from the ZIKA RNA.