What Does Functional Annotation in Metagenomic Analysis Involve

Question

What does functional annotation in metagenomic analysis involve?
A) determining the number of species in the sample
B) calculating the %G+C in the metagenome
C) finding homologs or recurring peptide motifs that infer the function of the sequence
D) making contigs and scaffolds and assembling the sequence
E) analyzing the RNA sequences to see what was transcribed

Accepted Answer

Functional annotation in metagenomic analysis involves finding homologs or recurring peptide motifs that infer the function of the sequence. This can be done through comparison with known protein or nucleotide databases, pathway analysis, and gene ontology analysis. It does not involve determining the number of species in the sample, calculating the %G+C in the metagenome, making contigs and scaffolds, or analyzing RNA sequences.