Question 9

(Multiple Choice)

The lambda genome is replicated by a rolling circle mechanism. What is the end result after a round of replication?


A) a single-stranded DNA that is immediately packaged in the phage head
B) two molecules resulting from a step of circle-to-circle replication
C) several circular molecules inserted into each other; the circles are cleaved at the cos sites and eventually relegated
D) a concatemer (i.e., multiple genomes joined end to tail, which are cleaved at cos sites by a terminase), producing linear double-stranded DNA prior to packaging
E) several Okazaki fragments that are subsequently ligated

Answer