The figure below diagrams a process used to study bacterial physiology. Which is the correct description of the steps shown?

A) Cells are lysed, two-dimensional gels are run, and then spots of protein are run through mass spec. Proteins' peaks are identified.
B) Cells are lysed, two-dimensional gels are run, isobaric tags are added to the proteins, and they are run through a mass spec. Proteins' peaks are identified.
C) Cells are lysed, two-dimensional gels are run, and proteins are digested with trypsin and run through a mass spec. Proteins' peaks are identified.
D) Cells are lysed, proteins are digested with trypsin, and column is used followed by mass spec. Proteins' peaks are identified.
E) Cells are lysed, RNA is purified, cDNA is made, and sequencing is carried out.