Which of the Following is a Technique Used in Studying

Question

Which of the following is a technique used in studying the proteome of an organism?
A) knowing the DNA sequence to predict the amino acid sequence
B) predicting the protein sequence by using the mRNA sequence
C) two-dimensional protein gels followed by mass spec of the proteins
D) obtaining cDNA and then probing a DNA microarray
E) digesting a cell extract with trypsin

Accepted Answer

Two-dimensional protein gels followed by mass spectrometry is a technique used to separate and identify the different proteins present in a sample, allowing for the study of the proteome of an organism. Options A and B are methods for predicting protein sequences but not for directly studying the proteome. Option D is a method for studying gene expression, not the proteome. Option E is a method for digesting proteins but not for studying the entire proteome of an organism.