Which of the Following Describes the Process of Obtaining RNA

Question

Which of the following describes the process of obtaining RNA sequence information?
A) Proteins are isolated and mass spec is used. Once the proteins are known, the RNA that coded for them can be determined.
B) RNA from a particular growth condition is isolated and converted to cDNA; DNA adapters are added, allowing high-throughput sequencing to be carried out. The genome position of the sequence obtained is then determined using computer software.
C) A DNA microarray is used to find the RNA sequences expressed at that time.
D) One examines the tRNA and rRNA in a given culture in order to study various physiological conditions.
E) One determines the RNA sequence of every gene in the genome and then determines if it is present in a given culture.

Accepted Answer

Option B describes the process of obtaining RNA sequence information by isolating RNA from a particular growth condition, converting it to cDNA, adding DNA adapters and then using high-throughput sequencing. The genome position of the obtained sequence is then determined using computer software. This is a common method for obtaining RNA sequence information. The other options do not describe this process accurately.