Agarose Gel Electrophoresis Separates Pieces of Linear DNA Based On

Question

Agarose gel electrophoresis separates pieces of linear DNA based on
A) size.
B) charge.
C) sequence.
D) degree of supercoiling.
E) extent of methylation.

Accepted Answer

Agarose gel electrophoresis separates pieces of linear DNA based on their size. The smaller DNA fragments move faster through the gel and further down the gel towards the positive electrode, while larger fragments move slower and remain closer to the well. The charge of the DNA does not play a significant role in this process. Sequence, degree of supercoiling, and extent of methylation are also not factors in agarose gel electrophoresis.