Which of These Techniques Would Provide the Best Resolution of an Enzyme's

Question

Which of these techniques would provide the best resolution of an enzyme's structure?
A) scanning electron microscopy
B) transmission electron microscopy
C) cryo-EM
D) X-ray diffraction analysis
E) atomic force microscopy

Accepted Answer

X-ray diffraction analysis is the best technique for determining the structure of enzymes at atomic resolution. It involves shining X-rays through a crystal of the enzyme and analyzing the diffraction pattern produced. This technique has been used to solve the structures of thousands of enzymes and is often considered the gold standard for determining protein structures.