In Which Type of Microscopy Do Dust Particles Interfere the MOST

Question

In which type of microscopy do dust particles interfere the MOST?
A) bright-field
B) dark-field
C) phase-contrast
D) interference
E) fluorescence

Accepted Answer

Dark-field microscopy relies on using oblique illumination to create contrast in the sample, making the dust particles more visible. In contrast, bright-field microscopy illuminates the sample from below, and phase-contrast microscopy uses phase shifts to create contrast in the sample, so dust particles may not be as prominent. Interference microscopy and fluorescence microscopy use specific wavelengths of light to visualize the sample, so dust particles may not have as significant of an impact.