Which Type of Microscopy is Used to Identify the 3-D

Question

Which type of microscopy is used to identify the 3-D structure of biofilms?
A) phase-contrast
B) dark-field
C) florescent
D) bright-field
E) confocal

Accepted Answer

Confocal microscopy is used to identify the 3-D structure of biofilms. Confocal microscopy uses a laser and pinhole aperture to generate high-resolution images of fluorescently-labeled specimens, which can be reconstructed into three-dimensional images of the biofilm. The other microscopy techniques listed are not as effective at imaging three-dimensional structures.